UV-Visible Spectroscopy and Circular Dichroism Spectroscopy

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1 - Introduction to the course

1 - Introduction.mp4

03:43

2 - UVVisible Spectroscopy

2 - Basic concept of UVVisible Spectroscopy.mp4

02:49

3 - Interactions of organic molecules with UVVisible light.mp4

06:30

4 - What do we look in UVVisible spectrum.mp4

01:02

5 - UVVisible spectrum of ATP.mp4

01:36

6 - UVVisible spectrum of NADNADH.mp4

03:03

7 - UVVisible spectrum of Cytochrome c.mp4

01:20

8 - UVVisible spectrum of BetaCarotene.mp4

01:08

9 - UVVisible spectrum of proteins.mp4

01:27

10 - UVVisible spectroscopy and melting point of DNA.mp4

01:59

11 - UVVisible spectroscopy and purity of DNA and proteins.mp4

02:00

12 - Reference readings.html

3 - Circular Dichroism Spectroscopy

13 - Basic BeerLambert law.mp4

02:00

14 - Types of lights Unpolarized plane polarized and circularly polarized light.mp4

01:40

15 - What is circular dichroism.mp4

02:37

16 - How do we measure circular dichroism.mp4

03:47

17 - Ellipticity as the measurement of circular dichroism.mp4

05:27

18 - Circular dichroism of proteins 1.mp4

02:47

19 - Circular dichroism of proteins 2.mp4

02:53

20 - Circular dichroism of proteins 3.mp4

03:49

21 - Circular dichroism of DNA.mp4

03:19

22 - References to read circular dichroism spectroscopy.html

4 - Additional learning Ramachandran Plot for proteins

23 - Ramachandran plot.mp4

16:44

Description

Spectroscopy

What You'll Learn?

Basic Concept of UV-Visible spectroscopy.
Interaction of organic molecules with light and what do we measure in UV-Visible spectroscopy.
UV-Visible spectrum of ATP
UV-visible spectrum of NAD+/NADH
UV-Visible spectrum of cytochrome-c
UV-Visible spectrum of Beta-carotene
UV-Visible spectrum of Proteins
UV-Visible spectrum and melting point of DNA
UV-Visible spectrum and purity of DNA and proteins
Types of light and what is circular dichroism?
How are we measuring circular dichroism and introduction various parameters.
Circular dichroism of proteins
Circular dichroism of DNA
Additional learning on Ramachandran plot of proteins

Who is this for?

Bachelor's students of biology, organic chemistry, biochemistry and life sciences.

Master's students of biology, organic chemistry, biochemistry and life sciences.

Researchers in the field of biology, organic chemistry, biochemistry and life sciences.

Medical students and medical professionals

Biology, organic chemistry, biochemistry and life science enthusiasts

What You Need to Know?

No previous skills are required

Enthusiasm to learn biology and techniques related to biology, biochemistry and organic chemistry

More details

Description
The major issues with the courses on spectroscopy are inclusion of too much instrumentation, electronics, mathematicalÂ calculations, and very less discussion on data analysis. This course on UV-Visible spectroscopy and circular dichroism spectroscopy for the students is for the students who want to learn the basic aspects and the data analysis by using so many examples.
This course will be teaching about two major and important techniques related to biology and organic chemistry that is UV visible spectroscopy and circular dichroism spectroscopy. It is a perfect introductory course for the students which starts its journey with discussion on basic concept of visible spectroscopy and it tells you about how different organic molecules with double bonds, with conjugated systems, and with lone pairs are interacting with light. Then it starts discussing about different examples of UV visible spectroscopy data analysis with the help of graphs.
In the third section, it talks about circular dichroism by starting with types of light and what is actually circular dichroism. Then it tells you how we are measuring CD, circular dichroism, what is ellipticity, basic formulas, basic discussion of fundamentals, and basic mathematics of CD. Then it goes on and talks about circular dichroism of proteins by taking 3 to 4 examples. Then finally, the third section ends with circular dichroism of DNA.
This course also contains a additional learning section where you learn about a very interesting but complicated topic, The Ramachandran plot of protein secondary structures. So, guys, let us enjoy the whole discussion on UV visible spectroscopy and circular dichroism spectroscopy. All the best.
Who this course is for:
Bachelor's students of biology, organic chemistry, biochemistry and life sciences.
Master's students of biology, organic chemistry, biochemistry and life sciences.
Researchers in the field of biology, organic chemistry, biochemistry and life sciences.
Medical students and medical professionals
Biology, organic chemistry, biochemistry and life science enthusiasts

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