Introduction to Flow Cytometry

Focused View

Lavinia Ionescu

2:10:27

128 View

1 - Theoretical foundations of flow cytometry

1 - Introduction.mp4

02:48

1 - Spectral characteristics of phycoerythrin PE.html

2 - What Is Cytometry and What Does It Do.mp4

09:58

3 - Fluorescence Overview.mp4

04:14

4 - Excitation and Emission Spectra.mp4

06:01

4 - Online-Fluorescence-Spectra-Viewers.pdf

2 - Flow Cytometers

5 - The Components of a Flow Cytometer.mp4

04:57

6 - Fluidics.mp4

06:16

7 - Optics.mp4

10:33

8 - Electronics.mp4

04:14

8 - Flow-Cytometry-Analysis-Software.pdf

9 - Quality Control.mp4

03:58

10 - Maintenance.mp4

04:11

3 - Working with Fluorochromes

11 - Fluorochrome Overview.mp4

06:32

12 - Relative Brightness.mp4

04:14

13 - Multicolour Flow Cytometry.mp4

06:10

14 - Compensation.mp4

14:50

15 - Spreading.mp4

09:52

4 - Experiment Planning

16 - Experiment Preparation General Guidelines.mp4

06:41

17 - Experimental Controls.mp4

09:49

18 - Counting and Titration.mp4

02:47

5 - Data Acquisition and Analysis

20 - Scaling Plots and Gates.mp4

06:12

21 - Example Workflow and Good Practice Gating.mp4

06:10

Description

A flow cytometry primer

What You'll Learn?

Understand the fundamental principles of flow cytometry
Identify the components of a flow cytometer and describe their role
Interpret instrument quality control data
Understand how to determine what fluorochromes can be detected by a flow cytometer based on fluorochrome spectra and instrument configuration
Summarize characteristics of fluorochromes that are highly relevant in practice (single/tandem, brightness, fluorescence spillover and spreading potential)
Gain a working understanding of fluorescence compensation
Plan a flow cytometry experiment using adequate controls
Learn common first steps for troubleshooting

Who is this for?

Beginner or occasional users of flow cytometry.

What You Need to Know?

No flow cytometry experience needed.

More details

Description
This course is designed to help beginner or occasional users of flow cytometry become more familiar with the numerous facets of the field. It may also be useful to intermediate or more frequent operators as a review of the concepts that remain firmly in place across platforms: from the basics of light scattering and fluorescence to the universal main components of flow cytometers, the practical principles of multicolor compensation, and the roles of experimental controls typically used in flow cytometry.

The instructor has 15 years of experience in flow cytometry, at first in academia as a biomedical researcher, then in the industry as a flow cytometry application scientist.
The lectures are built with the awareness that flow cytometry is used for numerous applications, almost all of which rely upon detection of fluorescent molecules. Moreover, in today's "flow" landscape, one-laser instruments coexist with multi-laser analyzers capable of detecting dozens of colors, and several powerful options are available in terms of analysis software.
The course is not focused on any specific software or instruments and is not intended to substitute hands-on training and/or certification courses. Instead, it aims to present the elements that constitute a shared ground among platforms and provide a springboard to advanced flow cytometry concepts.

Captions (November 6, 2022 update): The course instructor is in the process of writing and adding captions; 9 of 21 videos have English captions.
Who this course is for:
Beginner or occasional users of flow cytometry.

User Reviews

Rating

average 0

Total votes0

Focused display

Data Science

Lavinia Ionescu

Instructor's Courses

I started using flow cytometry in 2007 as a postgraduate researcher and it quickly became the cornerstone of my first-author publications. Since then, I have operated, taught, and consulted in the field virtually throughout my career, including as a research associate in a university flow cytometry core facility and as a flow cytometry application scientist in the industry, and I have used a variety of applications, instruments, and software.

Udemy

View courses Udemy

Students take courses primarily to improve job-related skills.Some courses generate credit toward technical certification. Udemy has made a special effort to attract corporate trainers seeking to create coursework for employees of their company.