High Performance Liquid Chromatography (HPLC)

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Aydan Ozden

3:41:00

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1 - Introduction

1 - 1-1-PDF-INTRO.pdf

1 - Introduction.mp4

05:41

2 - Introduction to High Performance Liquid Chromatography HPLC

2 - 2-HPLC-I.pdf

2 - Introduction to High Performance Liquid Chromatography HPLC.mp4

03:25

3 - Chromatography

3 - 3-HPLC-C.pdf

3 - Chromatography.mp4

09:14

4 - Chromatography Principles

4 - 4-HPLC-CP.pdf

4 - Chromatography Principles.mp4

19:19

5 - Types of Chromatography

5 - 5-HPLC-TC.pdf

5 - Types of Chromatography.mp4

14:05

6 - Instrumental Design of HPLC System

6 - 6-1-HPLC-ID.pdf

6 - Simple Technical Design.mp4

09:00

7 - 6-2-HPLC-ID.pdf

7 - Technical Design.mp4

04:57

8 - 6-3-HPLC-ID.pdf

8 - Equipment Compartments.mp4

09:34

9 - 6-4-HPLC-ID-SR.pdf

9 - Solvent Reservoir.mp4

08:26

10 - 6-5-HPLC-ID-P.pdf

10 - Solvent Delivery System Pump.mp4

06:41

11 - 6-6-HPLC-ID-SU.pdf

11 - Sampler Unit Injector Autosampler.mp4

03:34

12 - 6-7-HPLC-ID-C.pdf

12 - Column.mp4

08:54

13 - 6-8-HPLC-ID-D.pdf

13 - Dedector.mp4

07:42

14 - 6-9-HPLC-ID-C.pdf

14 - PC Monitor.mp4

01:43

7 - HPLC Analysis

15 - 7-1-HPLCINANALYTICALCHEMISTRY.pdf

15 - HPLC in Analytical Chemistry.mp4

04:48

16 - 72.mp4

06:46

16 - Qualitative Analysis.mp4

06:46

17 - 7-3-QUANTITATIVEANALYSIS.pdf

17 - Quantitative Analysis.mp4

05:30

18 - 7-4-QUANTITATIVEANALYSISEXAMPLE.pdf

18 - Quantitative Analysis Example.mp4

09:08

8 - Types of HPLC

19 - 8-1-HPLC-TH.pdf

19 - Types of HPLC.mp4

00:31

20 - 82.mp4

06:27

20 - Normal Phase HPLC NP HPLC.mp4

06:27

21 - 8-3-HPLC-RP.pdf

21 - Reversed Phase HPLC RP HPLC.mp4

09:42

22 - 8-4-HPLC-IEX.pdf

22 - Ion Exchange Chromatography IEX.mp4

03:18

23 - Size Exclusion Chromatography SEC.mp4

05:43

9 - Descriptors of HPLC Analytical Method Development

24 - 9-1-HPLC-DH.pdf

24 - Retention Time.mp4

08:45

25 - 9-2-HPLC-DH.pdf

25 - Void Volume Dead Volume.mp4

04:01

26 - 9-3-HPLC-DH.pdf

26 - Retention Factor.mp4

06:46

27 - 9-4-HPLC-DH.pdf

27 - Selectivity.mp4

06:30

28 - 9-5-HPLC-DH.pdf

28 - Efficiency Teorical Plate Number.mp4

03:32

29 - 9-6-HPLC-DH.pdf

29 - Selectivity Efficiency.mp4

05:47

30 - 9-7-HPLC-DH.pdf

30 - Resolution.mp4

01:46

10 - Conclusion

31 - CONCLUSION-AYDANOZDEN.pdf

31 - Conclusion.mp4

06:32

Description

The Comprehensive Course to Become Professional in High Performance Liquid Chromatography (HPLC)

What You'll Learn?

Understand and define basics of chromatography by learning stationary phase, mobile phase and analyte
Explain chromatography principles by understanding of affinity theory (solubility & adsorption)
Explain the types of chromatography based on bed type, separation mechanism, and physical states of phases
Understand instrumental design of HPLC system by explaining HPLC compartments
Define mission and importance of HPLC compartments such as solvent reservoir, solvent delivery system (pump), sampler unit & injector, column, dedector, monitor
Evaluate mission of HPLC techniques in analytical chemistry by explaining qualitative and quantitative analysis
Define types of HPLC such as Normal Phase (NP HPLC), Reversed Phase (RP HPLC), Ion Exchange (IEX) and Size Exclusion Chromatography (SEC)
Evaluate and understand descriptors of HPLC such as Retention Time, Void Volume, Retention Factor, Selectivity, Efficiency (Teorical Plate Number), Resolution

Who is this for?

Anyone who is willing to learn High Performance Liquid Chromatography (HPLC)

Anyone who wants to learn the chemistry of the High Performance Liquid Chromatography (HPLC)

Anyone who wants to be qualified in analytical chemistry, instrumental analysis and pharmaceutical industry

Anyone who need to use HPLC techniques in analytical chemistry and/or pharmacy in the learning process

Anyone who desire to be master in HPLC analysis by using technical/chemical background

Anyone who wants to get certification related with High Performance Liquid Chromatography (HPLC)

More details

Description
High Performance Liquid Chromatography (HPLC) techniques are so popular and common in pharma industry, cosmetic industry, chemistry industry, food industry, polymer industry and environment laboratories.
High performance liquid chromatography (HPLC) is the crucial and integral analytical tool applied in all stages of drug discovery, development, and commercialization in the pharmaceutical industry, especially. At the each step of drug development, drug substances, ingredients and drug products are controlled and monitored by using HPLC techniques.
In this course, chromotography, chromatography principles, types of chromatography, instrumental design of HPLC, HPLC Analysis, Types of HPLC and Descriptors of HPLC have been discussed within all technical details.
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) COURSE SCOPE
1 INTRODUCTION
2 INTRODUCTION TO HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
3 CHROMATOGRAPHY
3_1 History of Chromatography
3_2 What is Chromatography?
3_3 What is Stationary Phase?
3_4 What is Mobile Phase?
3_5 What is Analyte?
4 CHROMATOGRAPHY PRINCIPLES
4_1 Chromatographic Seperation
4_2 Chromatography (Affinity & Interaction)
4_3 Chromatography (Solubility & Adsorption)
4_4 Chromatography (Solubility & Polarity)
4_5 Chromatography (Solubility & Hidrophilic / Hidrophobic)
4_6 Let's Categorize Molecules!
5 TYPES OF CHROMATOGRAPHY
5_1 Chromatography Classification Based on Bed (Column Chromatography, Paper Chromatogrphy, Thin Layer Chromatography)
5_2 Chromatography Classification Based on Separation Mechanism (Affinity Chromatography, Ion Exchange Chromatography, Gel Filtration Chromatography)
5_3 Chromatography Classification Based on Physical States of Phases (Gas Chromatography, Liquid Chromatography)
6 INSTRUMENTAL DESIGN OF HPLC SYSTEM
6_1 Instrumental Design of HPLC System_Simple Technical Design
6_2 Instrumental Design of HPLC System_Technical Design
6_3 Instrumental Design of HPLC System_Equipment Compartments
6_4 Instrumental Design of HPLC System_Solvent Reservoir
6_5 Instrumental Design of HPLC System_Solvent Delivery System (Pump)
6_6 Instrumental Design of HPLC System_Sampler Unit & Injector & Autosampler
6_7 Instrumental Design of HPLC System_Column
6_8 Instrumental Design of HPLC System_Dedector
6_9 Instrumental Design of HPLC System_PC Monitor
7 HPLC ANALYSIS
7_1 HPLC in Analytical Chemistry
7_2 Qualitative Analysis
7_3 Quantitative Analysis
7_4 Quantitative Analysis Example
8 TYPES OF HPLC
8_1 Types of HPLC
8_2 Normal Phase HPLC (NP HPLC)
8_3 Reversed Phase HPLC (RP HPLC)
8_4 Ion Exchange Chromatography (IEX)
8_5 Size Exclusion Chromatography (SEC)
9 DESCRIPTORS OF HPLC & ANALYTICAL METHOD DEVELOPMENT
9_1 Retention Time
9_2 Void Volume (Dead Volume)
9_3 Retention Factor
9_4 Selectivity
9_5 Efficiency (Teorical Plate Number)
9_6 Selectivity & Efficiency
9_7 Resolution
10 CONCLUSION

Who this course is for:
Anyone who is willing to learn High Performance Liquid Chromatography (HPLC)
Anyone who wants to learn the chemistry of the High Performance Liquid Chromatography (HPLC)
Anyone who wants to be qualified in analytical chemistry, instrumental analysis and pharmaceutical industry
Anyone who need to use HPLC techniques in analytical chemistry and/or pharmacy in the learning process
Anyone who desire to be master in HPLC analysis by using technical/chemical background
Anyone who wants to get certification related with High Performance Liquid Chromatography (HPLC)

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She is Aydan OZDEN. She is working as R&D Formulation Development Scientist at Pharmaceutical Company. She hold a Bachelor of Chemistry and a Master’s degree in Organic Chemistry. She offer proficiency in pharmaceutical Quality and Research and Development including but not limited to drug formulation development, drug manufacturing process, and associated scientific and regulatory elements. She has an extensive knowledge in pharmaceutical R&D, specially in the drug formulation development, drug manufacturing process and associated scientific and regulatory elements with 9 years experience. She also gained strong theoretical and practical background in conducting preformulation studies, formulation development, process optimization and scale up of generic drug products while knowing and applying main principles of medicinal chemistry.She has five Udemy courses presented below.- Quality in Pharmaceutical Industry (GMP & GLP & GHP): This course is strongly recommended for learners who interest pharmaceutical industry and desire to take a part in Quality, Production, Technology Transfer and Research&Development Departments.- Pharmaceutical Dosage Forms & Basics of Pharma Industry: This course is strongly recommended for learners who interest pharmaceutical industry, especially for fresh graduate, and desire to take a part in Quality, Production, Technology Transfer, Research & Development Departments and Business Development.- Pharmaceutical Manufacturing & Technology (GRANULATION): This course is strongly recommended for learners who interest pharmaceutical drug manufacturing and desire to take a part in Quality Assurance, Production, Technology Transfer and Research & Development Departments.- Pharmaceutical Manufacturing & Technology (COATING): This course is strongly recommended for learners who interest pharmaceutical drug manufacturing and desire to take a part in Quality Assurance, Production, Technology Transfer and Research & Development Departments.- Preformulation Studies for Pharmaceutical Industry: This course is strongly recommended for learners who interest physicochemical properties of drug product and desire to take a part in Research & Development Department.- Preparation of Pharmaceutical Development Part for CTD: This course is strongly recommended for learners who interest pharmaceutical development / drug development and desire to take a part in Research & Development, Technology Transfer and Regulatory Affairs Departments.

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